An Account of Amoeba discoides; its Culture
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چکیده
منابع مشابه
Cytoplasmic DNA-containing bodies and the response of amoebae to dimidium bromide.
The growth of Amoeba proteus (T1P) and Amoeba discoides (T1D) in the trypanocidal phenanthridinium, dimidium bromide was examined. At concentrations of drug between 2 and 4 /ig/ ml, A. proteus divided twice before inhibition and death. A. discoides was able to undergo an additional cycle of division before death. At other concentrations there were no differences in their response. Heterotransfe...
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Antrycide is an aminoquinaldine whose inhibitory action on the growth of Trypanosoma and Crithidia is not fully understood at the cellular level. The growth of Amoeba discoides in concentrations of antrycide between 0.5 and 2 microgram/ml was reduced considerably, while cells failed to divide in 4 microgram/ml. The effects on growth rate were reversible at least up until 7 days in antrycide. In...
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Responses to the introduction of heterologous cytoplasm and the fate of foreign organelles were investigated in amoebae. Heterologous cytoplasm was transferred by microinjection from Pelomyxa carolinensis to Amoeba discoides. In control experiments, homologous cytoplasm was transferred from one A. discoides to another. Recipient cells were observed by light microscopy, and samples were prepared...
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The microinjection of cytoplasm taken from one strain of large free-living amoeba into another strain is followed by an incompatibility phenomenon, the inhibition of division amongst the recipient cells. The post-microsomal supernatant fraction from Amoeba discoides (T1D13) injected into A. proteus (T1P) inhibited division in 90% of the injected cells. Further centrifugation of this fraction yi...
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1. A description is given of a method for replacing the original nucleus of an amoeba by a nucleus from another amoeba. This method has been used to study transfers of nuclei within a species (homotransfers) and between species (heterotransfers). The species used were A. proteus and A. discoides. 2. Removal of the nucleus from an amoeba inactivates the animal, so that movement is sporadic and u...
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